Abstract
Crystals of the Fab' fragment from the monoclonal anti-peptide antibody B1312 and of the Fab'-peptide antigen complex have been characterized. The monoclonal antibodies were raised against a synthetic homologue of the C-helix of myohemerythrin (residues 69-87 in myohemerythrin). The Fab'-peptide complex crystallizes in space group P6322 with unit cell dimensions a = b = 142.5 A, c = 101.5 A, alpha = beta = 90 degrees, gamma = 120 degrees, and Z = 1. The native Fab' crystallizes in space group P212121 with unit cell dimensions a = 98.0 A, b = 151.7 A, c = 80.8 A, alpha = beta = gamma = 90 degrees, and Z = 2. Both crystal forms diffract to beyond 2.6 A resolution. We also report the cDNA and predicted amino acid sequences for the variable regions of both the light and heavy chains of this anti-peptide antibody.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Antibodies, Monoclonal* / genetics
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Antigen-Antibody Complex* / isolation & purification
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Base Sequence
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Crystallization
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Hemerythrin* / analogs & derivatives
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Hemerythrin* / immunology
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Immunoglobulin Fab Fragments* / genetics
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Immunoglobulin Fab Fragments* / isolation & purification
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Immunoglobulin G / genetics
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Immunoglobulin G / isolation & purification
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Immunoglobulin Heavy Chains / genetics
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Immunoglobulin Light Chains / genetics
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Metalloproteins* / analogs & derivatives
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Metalloproteins* / immunology
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Molecular Sequence Data
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Peptide Fragments* / isolation & purification
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Protein Conformation
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X-Ray Diffraction
Substances
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Antibodies, Monoclonal
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Antigen-Antibody Complex
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Hemerythrin
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Immunoglobulin Fab Fragments
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Immunoglobulin G
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Immunoglobulin Heavy Chains
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Immunoglobulin Light Chains
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Metalloproteins
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Peptide Fragments
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myohemerythrin
Associated data
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GENBANK/M29250
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GENBANK/M29252