Interleukin 22 protects colorectal cancer cells from chemotherapy by activating the STAT3 pathway and inducing autocrine expression of interleukin 8

Clin Immunol. 2014 Oct;154(2):116-26. doi: 10.1016/j.clim.2014.07.005. Epub 2014 Jul 23.

Abstract

Resistance to chemotherapy is the major cause of colorectal cancer (CRC) treatment failure. The cytokine IL-22, which is produced by T cells and NK cells, is associated with tumorigenesis and tumor progression in cancers. However, the role of IL-22 in chemoresistance has not been investigated. We found that IL-22 levels in tumor tissues and peripheral blood were associated with chemoresistance and indicate poor prognosis for patients who received FOLFOX chemotherapy. In CRC cells, IL-22 was able to attenuate the cytotoxic and apoptosis-inducing effects of 5-FU and OXA by activating the STAT3 pathway and subsequently increasing the expression of anti-apoptotic genes. In addition, IL-22 conferred resistance to 5-FU and OXA by inducing IL-8 autocrine expression through STAT3 activation. Our findings identify IL-22 as a novel chemoresistance cytokine and may be a useful prognostic biomarker for CRC patients receiving FOLFOX chemotherapy.

Keywords: Chemoresistance; Interleukin 22; Interleukin 8; STAT3.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / therapeutic use*
  • Antineoplastic Combined Chemotherapy Protocols / therapeutic use
  • Autocrine Communication
  • Cell Line, Tumor
  • Colorectal Neoplasms / drug therapy*
  • Drug Resistance, Neoplasm
  • Fluorouracil / therapeutic use
  • Gene Expression Regulation, Neoplastic / physiology*
  • Humans
  • Interleukin-22
  • Interleukin-8 / genetics
  • Interleukin-8 / metabolism*
  • Interleukins / genetics
  • Interleukins / metabolism*
  • Leucovorin / therapeutic use
  • Organoplatinum Compounds / therapeutic use
  • STAT3 Transcription Factor / genetics
  • STAT3 Transcription Factor / metabolism*
  • Treatment Failure

Substances

  • Antineoplastic Agents
  • Interleukin-8
  • Interleukins
  • Organoplatinum Compounds
  • STAT3 Transcription Factor
  • STAT3 protein, human
  • Leucovorin
  • Fluorouracil

Supplementary concepts

  • Folfox protocol