Design and fabrication of biological wires

Methods Mol Biol. 2014:1181:157-65. doi: 10.1007/978-1-4939-1047-2_14.

Abstract

Cardiac tissue engineering using human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) has facilitated the creation of in vitro diagnostic platforms to study novel small molecules and cardiac disease at the tissue level. Yet, due to the immaturity of hPSC-CMs, there is a low fidelity between tissue-engineered cardiac tissues and adult cardiac tissues. To address this challenge, we have developed a platform that combines both physical and electrical cues to guide hPSC-CMs towards a more mature state in vitro.

MeSH terms

  • Animals
  • Cell Differentiation / drug effects
  • Cell Separation
  • Collagen Type I / pharmacology
  • Cytological Techniques / instrumentation*
  • Dimethylpolysiloxanes / pharmacology
  • Electric Stimulation
  • Equipment Design
  • Humans
  • Myocytes, Cardiac / cytology
  • Pluripotent Stem Cells / cytology*
  • Pluripotent Stem Cells / drug effects
  • Rats
  • Tissue Engineering*

Substances

  • Collagen Type I
  • Dimethylpolysiloxanes
  • baysilon