Dynamics of genomic H3K27me3 domains and role of EZH2 during pancreatic endocrine specification

EMBO J. 2014 Oct 1;33(19):2157-70. doi: 10.15252/embj.201488671. Epub 2014 Aug 8.

Abstract

Endoderm cells undergo sequential fate choices to generate insulin-secreting beta cells. Ezh2 of the PRC2 complex, which generates H3K27me3, modulates the transition from endoderm to pancreas progenitors, but the role of Ezh2 and H3K27me3 in the next transition to endocrine progenitors is unknown. We isolated endoderm cells, pancreas progenitors, and endocrine progenitors from different staged mouse embryos and analyzed H3K27me3 genome-wide. Unlike the decline in H3K27me3 domains reported during embryonic stem cell differentiation in vitro, we find that H3K27me3 domains increase in number during endocrine progenitor development in vivo. Genes that lose the H3K27me3 mark typically encode transcriptional regulators, including those for pro-endocrine fates, whereas genes that acquire the mark typically are involved in cell biology and morphogenesis. Deletion of Ezh2 at the pancreas progenitor stage enhanced the production of endocrine progenitors and beta cells. Inhibition of EZH2 in embryonic pancreas explants and in human embryonic stem cell cultures increased endocrine progenitors in vitro. Our studies reveal distinct dynamics in H3K27me3 targets in vivo and a means to modulate beta cell development from stem cells.

Keywords: Ezh2; H3K27me3; embryogenesis; endocrine; pancreas.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Differentiation
  • Cell Lineage
  • Cells, Cultured
  • Chromatin Immunoprecipitation
  • Embryonic Stem Cells / cytology
  • Embryonic Stem Cells / metabolism
  • Endocrine Cells / cytology*
  • Endocrine Cells / metabolism
  • Enhancer of Zeste Homolog 2 Protein
  • Gene Expression Regulation, Developmental
  • Histones / genetics
  • Histones / metabolism*
  • Humans
  • Immunoenzyme Techniques
  • Integrases / metabolism
  • Islets of Langerhans / cytology*
  • Islets of Langerhans / metabolism
  • Jumonji Domain-Containing Histone Demethylases / genetics*
  • Mice
  • Mice, Knockout
  • Organogenesis
  • Polycomb Repressive Complex 2 / physiology*
  • RNA, Messenger / genetics
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stem Cells / cytology
  • Stem Cells / metabolism

Substances

  • Histones
  • RNA, Messenger
  • Jumonji Domain-Containing Histone Demethylases
  • Kdm6b protein, mouse
  • Enhancer of Zeste Homolog 2 Protein
  • Ezh2 protein, mouse
  • Polycomb Repressive Complex 2
  • Cre recombinase
  • Integrases