Successful off-label use of the Cepheid Xpert GBS in a late-onset neonatal meningitis by Streptococcus agalactiae

Int J Clin Exp Pathol. 2014 Jul 15;7(8):5192-5. eCollection 2014.

Abstract

We report the case of a late-onset neonatal meningitis by Streptococcus agalactiae (group B Streptococcus - GBS) that was diagnosed with a latex agglutination assay (on cerebrospinal fluid, CSF), as well as by using, for the first time, Xpert GBS (Cepheid, US) on CSF. Due to empirical antibiotics given before sampling, both CSF and blood culture were negative, so the abovementioned diagnostics was crucial. Moreover, the Xpert GBS assay, performed according to an off-label, modified protocol (the system is designed for GBS-carriage intrapartum screening, based on a completely automated real time-Polymerase Chain Reaction) quickly detected the organism's genome target. Although further investigation on this test's performace on CSF is required, then, we trust it may be a promising, quick and precise diagnostic method for infections in newborns.

Keywords: Cepheid; GBS; Lancefield group B; Streptococcus agalactiae; Xpert GBS; gestation; meningitis; pregnancy.

Publication types

  • Case Reports

MeSH terms

  • Bacterial Typing Techniques / instrumentation
  • Bacterial Typing Techniques / methods*
  • Humans
  • Infant
  • Male
  • Meningitis, Bacterial / diagnosis*
  • Real-Time Polymerase Chain Reaction / instrumentation
  • Real-Time Polymerase Chain Reaction / methods
  • Streptococcal Infections / diagnosis*
  • Streptococcus agalactiae