In Vitro selection of Neisseria gonorrhoeae mutants with elevated MIC values and increased resistance to cephalosporins

Steven R Johnson; Yonatan Grad; Satishkumar Ranganathan Ganakammal; Mark Burroughs; Mike Frace; Marc Lipsitch; Ryan Weil; David Trees

doi:10.1128/AAC.03082-14

In Vitro selection of Neisseria gonorrhoeae mutants with elevated MIC values and increased resistance to cephalosporins

Antimicrob Agents Chemother. 2014 Nov;58(11):6986-9. doi: 10.1128/AAC.03082-14. Epub 2014 Sep 8.

Authors

Steven R Johnson¹, Yonatan Grad², Satishkumar Ranganathan Ganakammal³, Mark Burroughs⁴, Mike Frace³, Marc Lipsitch⁵, Ryan Weil³, David Trees⁶

Affiliations

¹ Division of STD Prevention, NCHHSTP, Centers for Disease Control and Prevention, Atlanta, Georgia, USA [email protected].
² Center for Communicable Disease Dynamics, Department of Epidemiology, Harvard School of Public Health, Boston, Massachusetts, USA Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
³ Division of Scientific Resources, NCEZID, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
⁴ Division of STD Prevention, NCHHSTP, Centers for Disease Control and Prevention, Atlanta, Georgia, USA Division of Scientific Resources, NCEZID, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
⁵ Center for Communicable Disease Dynamics, Department of Epidemiology, Harvard School of Public Health, Boston, Massachusetts, USA Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA.
⁶ Division of STD Prevention, NCHHSTP, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

Abstract

Strains of Neisseria gonorrhoeae with mosaic penA genes bearing novel point mutations in penA have been isolated from ceftriaxone treatment failures. Such isolates exhibit significantly higher MIC values to third-generation cephalosporins. Here we report the in vitro isolation of two mutants with elevated MICs to cephalosporins. The first possesses a point mutation in the transpeptidase region of the mosaic penA gene, and the second contains an insertion mutation in pilQ.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Anti-Bacterial Agents / pharmacology
Cephalosporins / pharmacology*
Drug Resistance, Multiple, Bacterial
Erythromycin / pharmacology
Fimbriae Proteins / genetics*
Gonorrhea / drug therapy
Gonorrhea / microbiology
Humans
Microbial Sensitivity Tests
Molecular Sequence Data
Mutation
Neisseria gonorrhoeae / drug effects*
Neisseria gonorrhoeae / genetics*
Neisseria gonorrhoeae / isolation & purification
Penicillin-Binding Proteins / genetics
Treatment Failure
beta-Lactamases / genetics*

Substances

Anti-Bacterial Agents
Cephalosporins
Penicillin-Binding Proteins
pilQ protein, bacteria
Fimbriae Proteins
Erythromycin
beta-Lactamases

Associated data

GENBANK/KM403400
GENBANK/KM403401
GENBANK/KM452733

Abstract

Publication types

MeSH terms

Substances

Associated data

Grants and funding