Overexpressed miRNA-137 inhibits human glioma cells growth by targeting Rac1

Cancer Biother Radiopharm. 2013 May;28(4):327-34. doi: 10.1089/cbr.2012.1380.

Abstract

Previous studies have shown that miR-137 functions as a tumor suppressor in various cancers, but its role in the initiation and development of gliomas is still unknown. Currently, we found that miR-137 exhibited the most significant increase in normal brain tissues compared with glioma specimens, and the miR-137 expression was greatly decreased with the ascending of tumor pathological grades. Furthermore, overexpression of miR-137 in vitro by chemically synthesized miR-137 mimics suppressed the proliferation, inhibited cell cycle arrest in the G1/G0 phase, and induced cell apoptosis. The tumor-suppressive effects of miR-137 were indeed induced by Rac1, which was verified as a direct target of miR-137. These findings indicate that miR-137 inhibits the growth of gliomas cells by directly targeting Rac1, suggesting that miR-137 could be a new important therapeutic strategy for glioma treatment and warrants further investigation.

Keywords: Rac1; glioblastoma; miR-137.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis
  • Brain Chemistry
  • Cell Line, Tumor
  • Cell Proliferation
  • Central Nervous System Neoplasms / chemistry*
  • Central Nervous System Neoplasms / genetics
  • Central Nervous System Neoplasms / pathology*
  • Cyclin D1 / metabolism
  • G1 Phase Cell Cycle Checkpoints
  • Glioma / chemistry
  • Glioma / genetics
  • Glioma / metabolism*
  • Glioma / pathology*
  • Humans
  • Matrix Metalloproteinase 2 / metabolism
  • MicroRNAs / analysis
  • MicroRNAs / genetics
  • MicroRNAs / metabolism
  • MicroRNAs / physiology*
  • Neoplasm Grading
  • Neoplasm Invasiveness
  • Proto-Oncogene Proteins c-bcl-2 / metabolism
  • RNA, Small Interfering / genetics
  • Signal Transduction
  • Transfection
  • rac1 GTP-Binding Protein / metabolism*

Substances

  • MIRN137 microRNA, human
  • MicroRNAs
  • Proto-Oncogene Proteins c-bcl-2
  • RAC1 protein, human
  • RNA, Small Interfering
  • Cyclin D1
  • Matrix Metalloproteinase 2
  • rac1 GTP-Binding Protein