Molecular characterization of blaNDM-5 carried on an IncFII plasmid in an Escherichia coli isolate from a nontraveler patient in Spain

Antimicrob Agents Chemother. 2015 Jan;59(1):659-62. doi: 10.1128/AAC.04040-14. Epub 2014 Oct 13.

Abstract

A carbapenem-resistant Escherichia coli isolate (sequence type 448 [ST448]) was recovered from a urine culture of a female patient with no recent record of traveling. PCR screening identified the presence of bla(NDM-5), bla(TEM-1), bla(OXA-1), bla(CMY-42), and rmtB. bla(NDM-5) was carried in a conjugative IncFII-type plasmid (90 kb) together with bla(TEM-1) and rmtB. The genetic environment of bla(NDM-5) showed a structure similar to those of pMC-NDM and pGUE-NDM, identified in Poland and France in E. coli of African and Indian origin, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Bacterial Proteins / genetics
  • Escherichia coli / drug effects
  • Escherichia coli / genetics*
  • Escherichia coli Infections / drug therapy
  • Escherichia coli Infections / microbiology*
  • Escherichia coli Proteins / genetics
  • Female
  • Genes, Bacterial / genetics
  • Humans
  • Molecular Sequence Data
  • Plasmids / genetics
  • Spain / epidemiology
  • beta-Lactam Resistance / genetics*
  • beta-Lactamases / genetics

Substances

  • Bacterial Proteins
  • Escherichia coli Proteins
  • beta-Lactamases
  • carbapenemase

Associated data

  • GENBANK/KM598665