DPPX potassium channel antibody: frequency, clinical accompaniments, and outcomes in 20 patients

Neurology. 2014 Nov 11;83(20):1797-803. doi: 10.1212/WNL.0000000000000991. Epub 2014 Oct 15.

Abstract

Objective: To describe the detection frequency and clinical associations of immunoglobulin G (IgG) targeting dipeptidyl-peptidase-like protein-6 (DPPX), a regulatory subunit of neuronal Kv4.2 potassium channels.

Methods: Specimens from 20 patients evaluated on a service basis by tissue-based immunofluorescence yielded a synaptic immunostaining pattern consistent with DPPX-IgG (serum, 20; CSF, all 7 available). Transfected HEK293 cell-based assay confirmed DPPX specificity in all specimens. Sixty-nine patients with stiff-person syndrome and related disorders were also evaluated by DPPX-IgG cell-based assay.

Results: Of 20 seropositive patients, 12 were men; median symptom onset age was 53 years (range, 13-75). Symptom onset was insidious in 15 and subacute in 5. Twelve patients reported prodromal weight loss. Neurologic disorders were multifocal. All had one or more brain or brainstem manifestations: amnesia (16), delirium (8), psychosis (4), depression (4), seizures (2), and brainstem disorders (15; eye movement disturbances [8], ataxia [7], dysphagia [6], dysarthria [4], respiratory failure [3]). Nine patients reported sleep disturbance. Manifestations of central hyperexcitability included myoclonus (8), exaggerated startle (6), diffuse rigidity (6), and hyperreflexia (6). Dysautonomia involved the gastrointestinal tract (9; diarrhea [6], gastroparesis, and constipation [3]), bladder (7), cardiac conduction system (3), and thermoregulation (1). Two patients had B-cell neoplasms: gastrointestinal lymphoma (1), and chronic lymphocytic leukemia (1). Substantial neurologic improvements followed immunotherapy in 7 of 11 patients with available treatment data. DPPX-IgG was not detected in any of the stiff-person syndrome patients.

Conclusions: DPPX-IgG is a biomarker for an immunotherapy-responsive multifocal neurologic disorder of the central and autonomic nervous systems.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adrenal Cortex Hormones / therapeutic use
  • Adult
  • Aged
  • Autonomic Nervous System / pathology
  • Brain / pathology
  • Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / immunology*
  • Female
  • Gastrointestinal Diseases / etiology
  • HEK293 Cells
  • Humans
  • Immunoglobulin G / blood*
  • Immunoglobulin G / cerebrospinal fluid*
  • Immunotherapy
  • Magnetic Resonance Imaging
  • Male
  • Mental Disorders / etiology
  • Middle Aged
  • Nerve Tissue Proteins / immunology*
  • Nervous System Diseases / diagnosis*
  • Nervous System Diseases / metabolism
  • Nervous System Diseases / physiopathology*
  • Nervous System Diseases / therapy
  • Potassium Channels / immunology*
  • Sleep Wake Disorders / etiology
  • Transfection
  • Weight Loss / physiology
  • Young Adult

Substances

  • Adrenal Cortex Hormones
  • Immunoglobulin G
  • Nerve Tissue Proteins
  • Potassium Channels
  • DPP6 protein, human
  • Dipeptidyl-Peptidases and Tripeptidyl-Peptidases