Abstract
The intrinsic and extrinsic factors that contribute to stem and neuronal precursor cell maintenance and/or differentiation remain poorly understood. Proteoglycans, major residents of the stem cell microenvironment, modulate key signaling cues and are of particular importance. We have taken a loss-of-function approach, by developing a library of bacterial lyases and sulfatases to specifically remodel the ECM and test the functional role of glycosaminoglycans (GAGs) in cell self-renewal, maintenance, and differentiation.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Biochemistry / methods*
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Cell Differentiation
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Cell Proliferation
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Cerebral Cortex / metabolism
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Chondroitinases and Chondroitin Lyases / genetics
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Cloning, Molecular
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Electrophoresis
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Embryonic Stem Cells / cytology
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Embryonic Stem Cells / metabolism
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Epitopes / metabolism
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Fluorescent Dyes / chemistry
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Glycosaminoglycans / metabolism
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Glycoside Hydrolases / metabolism*
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Mice
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Molecular Sequence Data
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Protein Engineering
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Protein Sorting Signals
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Proteoglycans / metabolism*
Substances
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Epitopes
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Fluorescent Dyes
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Glycosaminoglycans
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Protein Sorting Signals
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Proteoglycans
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Glycoside Hydrolases
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Chondroitinases and Chondroitin Lyases