Super-resolution microscopy approaches for live cell imaging

Biophys J. 2014 Oct 21;107(8):1777-1784. doi: 10.1016/j.bpj.2014.08.028.

Abstract

By delivering optical images with spatial resolutions below the diffraction limit, several super-resolution fluorescence microscopy techniques opened new opportunities to study biological structures with details approaching molecular structure sizes. They have now become methods of choice for imaging proteins and their nanoscale dynamic organizations in live cells. In this mini-review, we describe and compare the main far-field super-resolution approaches that allow studying endogenous or overexpressed proteins in live cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Imaging, Three-Dimensional / methods*
  • Limit of Detection*
  • Microscopy, Fluorescence / methods
  • Single-Cell Analysis / methods