A lack of confirmation with alternative assays questions the validity of IL-17A expression in human neutrophils using immunohistochemistry

Immunol Lett. 2014 Dec;162(2 Pt B):194-8. doi: 10.1016/j.imlet.2014.10.025. Epub 2014 Oct 28.

Abstract

We identified IL-17A-positive neutrophils in Wolbachia-positive Onchocerca volvulus nodules using an antibody that has previously reported IL-17A-positive neutrophils in several inflammatory conditions. However, we could not detect IL-17A using a range of alternative assays. Our data question the IL-17A antibody specificity and the ability of human neutrophils to express IL-17A.

Keywords: IL-17A; Interleukin-17; Neutrophil; Wolbachia.

Publication types

  • Clinical Trial, Phase II
  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Helminth / chemistry*
  • Antibodies, Helminth / immunology
  • Female
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / immunology
  • Humans
  • Immunohistochemistry / methods
  • Interleukin-17 / immunology*
  • Male
  • Neutrophils / immunology*
  • Neutrophils / pathology
  • Onchocerca volvulus / immunology*
  • Onchocerciasis / drug therapy
  • Onchocerciasis / immunology*
  • Onchocerciasis / pathology

Substances

  • Antibodies, Helminth
  • IL17A protein, human
  • Interleukin-17