Genomic characterisation of three Mapputta group viruses, a serogroup of Australian and Papua New Guinean bunyaviruses associated with human disease

PLoS One. 2015 Jan 14;10(1):e0116561. doi: 10.1371/journal.pone.0116561. eCollection 2015.

Abstract

The Mapputta serogroup tentatively contains the mosquito-associated viruses Mapputta, Maprik, Trubanaman and Gan Gan. Interestingly, this serogroup has previously been associated with an acute epidemic polyarthritis-like illness in humans; however, there has been no ensuing genetic characterisation. Here we report the complete genome sequences of Mapputta and Maprik viruses, and a new Mapputta group candidate, Buffalo Creek virus, previously isolated from mosquitoes and detected by serology in a hospitalised patient. Phylogenetic analyses indicate that the group is one of the earliest diverged groups within the genus Orthobunyavirus of the family Bunyaviridae. Analyses show that these three viruses are related to the recently sequenced Australian bunyaviruses from mosquitoes, Salt Ash and Murrumbidgee. A notable feature of the Mapputta group viruses is the absence of the NSs (non-structural) ORF commonly found on the S segment of other orthobunyaviruses. Viruses of the Mapputta group have been isolated from geographically diverse regions ranging from tropical Papua New Guinea to the semi-arid climate of south-eastern Australia. The relevance of this group to human health in the region merits further investigation.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bunyaviridae Infections / virology*
  • Culicidae / virology
  • Genome, Viral / genetics*
  • Genomics / methods
  • Humans
  • Orthobunyavirus / genetics*
  • Papua-Neuguinea
  • Phylogeny
  • Sequence Analysis, DNA / methods
  • Serogroup
  • South Australia

Grants and funding

The authors received no specific funding for this work.