Deconstruction of archaeal genome depict strategic consensus in core pathways coding sequence assembly

PLoS One. 2015 Feb 12;10(2):e0118245. doi: 10.1371/journal.pone.0118245. eCollection 2015.

Abstract

A comprehensive in silico analysis of 71 species representing the different taxonomic classes and physiological genre of the domain Archaea was performed. These organisms differed in their physiological attributes, particularly oxygen tolerance and energy metabolism. We explored the diversity and similarity in the codon usage pattern in the genes and genomes of these organisms, emphasizing on their core cellular pathways. Our thrust was to figure out whether there is any underlying similarity in the design of core pathways within these organisms. Analyses of codon utilization pattern, construction of hierarchical linear models of codon usage, expression pattern and codon pair preference pointed to the fact that, in the archaea there is a trend towards biased use of synonymous codons in the core cellular pathways and the Nc-plots appeared to display the physiological variations present within the different species. Our analyses revealed that aerobic species of archaea possessed a larger degree of freedom in regulating expression levels than could be accounted for by codon usage bias alone. This feature might be a consequence of their enhanced metabolic activities as a result of their adaptation to the relatively O2-rich environment. Species of archaea, which are related from the taxonomical viewpoint, were found to have striking similarities in their ORF structuring pattern. In the anaerobic species of archaea, codon bias was found to be a major determinant of gene expression. We have also detected a significant difference in the codon pair usage pattern between the whole genome and the genes related to vital cellular pathways, and it was not only species-specific but pathway specific too. This hints towards the structuring of ORFs with better decoding accuracy during translation. Finally, a codon-pathway interaction in shaping the codon design of pathways was observed where the transcription pathway exhibited a significantly different coding frequency signature.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Biological
  • Archaea / genetics*
  • Archaea / metabolism*
  • Base Composition
  • Biological Transport
  • Cluster Analysis
  • Codon
  • Energy Metabolism
  • Gene Expression
  • Genome, Archaeal*
  • Genomics*
  • Metabolic Networks and Pathways*
  • Open Reading Frames*
  • Oxygen Consumption

Substances

  • Codon

Grants and funding

SM and RB received grants from the Department of Biotechnology, Govt. of India (http://dbtindia.nic.in) vide grant no. BT/BI/04/026/93 and BT/BI/010/019/99. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.