FZD2 inhibits the cell growth and migration of salivary adenoid cystic carcinomas

Oncol Rep. 2016 Feb;35(2):1006-12. doi: 10.3892/or.2015.3811. Epub 2015 Feb 19.

Abstract

Several studies have reported that FZD2 regulates tumor biology in a complex manner. The aim of the present study was to identify the role of FZD2 in the cell growth and metastasis of salivary adenoid cystic carcinomas (SACCs). The expression of FZD2 in ACC-83 and ACC-LM cells were measured with real-time PCR. Immunohistochemical staining was used to detect the expression of FZD2 in clinical SACC samples with or without metastasis. Cell proliferation and Transwell assays were performed to explore the effects of FZD2 on cell growth and migration following the silencing of FZD2 with small interference RNAs and the overexpression of FZD2 with plasmid. Our data showed that FZD2 was downregulated in ACC-LM cells, which are an adenoid cystic carcinoma cell line with high metastatic potential, compared to ACC-83 cells, which have low metastatic potential. Additionally, the expression of FZD2 was lower in SACC tissues with metastasis compared to SACC tissues without metastasis (P<0.05). Cell proliferation and migration of ACC-83 cells were increased after the knockdown of FZD2 and decreased following overexpression of FZD2. Knockdown of FZD2 downregulated the expression of PAI-1. Our results suggest that FZD2 may be a tumor suppressor gene in SACCs that inhibits cell growth and migration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma, Adenoid Cystic / pathology*
  • Cell Division
  • Cell Line, Tumor
  • Cell Movement
  • Down-Regulation
  • Frizzled Receptors / physiology*
  • Humans
  • Neoplasm Invasiveness
  • Neoplasm Proteins / physiology*
  • Plasminogen Activator Inhibitor 1 / physiology
  • RNA Interference
  • RNA, Small Interfering / genetics
  • Recombinant Fusion Proteins / metabolism
  • Salivary Gland Neoplasms / pathology*
  • Transfection
  • Tumor Stem Cell Assay
  • Wnt Signaling Pathway

Substances

  • FZD2 protein, human
  • Frizzled Receptors
  • Neoplasm Proteins
  • Plasminogen Activator Inhibitor 1
  • RNA, Small Interfering
  • Recombinant Fusion Proteins
  • SERPINE1 protein, human