Abstract
A number of CD4+ T cell lines were compared for their ability to act as target cells for human immunodeficiency virus (HIV) infection in syncytium- and plaque-forming assays. MT-4 and C8166 cells were the most sensitive indicator cells for HIV- and simian immunodeficiency virus (SIV)-induced cytopathic effects, and gave rise to macroscopic (MT-4) and microscopic (C8166) plaques. The MT-4 plaque assay was evaluated for the measurement of HIV- and SIV-neutralizing antibodies.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / analysis
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CD4-Positive T-Lymphocytes / microbiology*
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Cell Line
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Cytopathogenic Effect, Viral
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HIV / growth & development
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HIV / immunology
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HIV / physiology*
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HIV Antibodies / analysis
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Humans
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Immune Sera / immunology
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Neutralization Tests
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Simian Immunodeficiency Virus / growth & development
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Simian Immunodeficiency Virus / immunology
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Simian Immunodeficiency Virus / physiology*
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Viral Plaque Assay
Substances
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Antibodies, Viral
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HIV Antibodies
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Immune Sera