Prednisolone-containing liposomes accumulate in human atherosclerotic macrophages upon intravenous administration

Nanomedicine. 2015 Jul;11(5):1039-46. doi: 10.1016/j.nano.2015.02.021. Epub 2015 Mar 17.

Abstract

Drug delivery to atherosclerotic plaques via liposomal nanoparticles may improve therapeutic agents' risk-benefit ratios. Our paper details the first clinical studies of a liposomal nanoparticle encapsulating prednisolone (LN-PLP) in atherosclerosis. First, PLP's liposomal encapsulation improved its pharmacokinetic profile in humans (n=13) as attested by an increased plasma half-life of 63h (LN-PLP 1.5mg/kg). Second, intravenously infused LN-PLP appeared in 75% of the macrophages isolated from iliofemoral plaques of patients (n=14) referred for vascular surgery in a randomized, placebo-controlled trial. LN-PLP treatment did however not reduce arterial wall permeability or inflammation in patients with atherosclerotic disease (n=30), as assessed by multimodal imaging in a subsequent randomized, placebo-controlled study. In conclusion, we successfully delivered a long-circulating nanoparticle to atherosclerotic plaque macrophages in patients, whereas prednisolone accumulation in atherosclerotic lesions had no anti-inflammatory effect. Nonetheless, the present study provides guidance for development and imaging-assisted evaluation of future nanomedicine in atherosclerosis.

From the clinical editor: In this study, the authors undertook the first clinical trial using long-circulating liposomal nanoparticle encapsulating prednisolone in patients with atherosclerosis, based on previous animal studies. Despite little evidence of anti-inflammatory effect, the results have provided a starting point for future development of nanomedicine in cardiovascular diseases.

Keywords: Atherosclerosis; Glucocorticoids; Macrophages; Nanomedicine.

Publication types

  • Randomized Controlled Trial
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Intravenous
  • Adult
  • Aged
  • Anti-Inflammatory Agents / administration & dosage*
  • Anti-Inflammatory Agents / pharmacokinetics
  • Anti-Inflammatory Agents / therapeutic use
  • Arteries / drug effects
  • Arteries / pathology
  • Atherosclerosis / drug therapy*
  • Atherosclerosis / pathology
  • Female
  • Glucocorticoids / administration & dosage*
  • Glucocorticoids / pharmacokinetics
  • Glucocorticoids / therapeutic use
  • Humans
  • Liposomes
  • Macrophages / drug effects*
  • Macrophages / pathology
  • Male
  • Middle Aged
  • Plaque, Atherosclerotic / drug therapy*
  • Plaque, Atherosclerotic / pathology
  • Prednisolone / administration & dosage*
  • Prednisolone / pharmacokinetics
  • Prednisolone / therapeutic use

Substances

  • Anti-Inflammatory Agents
  • Glucocorticoids
  • Liposomes
  • Prednisolone