Role of microRNA-30c targeting ADAM19 in colorectal cancer

PLoS One. 2015 Mar 23;10(3):e0120698. doi: 10.1371/journal.pone.0120698. eCollection 2015.

Abstract

MicroRNAs (miRNAs) are deregulated in a number of cancers including colorectal cancer. MiR-30c belongs to miR-30 family, and is involved in a variety of malignant diseases. In this study, we detected the expression of miR-30c in colon cancer cell lines and clinical colon cancer specimens. MiR-30c was shown to be dramatically down-regulated both in cell lines and cancer tissues. Additionally, miR-30c could inhibit cancer cell growth, migration and invasion in vitro. Consistently, stable over-expression of miR-30c inhibited the growth and lung metastasis of colon cancer cell xenografts in vivo. Furthermore, bioinformatics algorithm and luciferase reporter assay indicated ADAM19 as a direct target of miR-30c. Of interest, further experiments demonstrated that inhibition of ADAM19 by miR-30c partially mediated the anti-tumor effect of miR-30c. Overall, our study provides the new insight that miR-30c inhibited colon cancer cells via targeting ADAM19. Thus, miR-30c might serve as a promising therapeutic strategy for colon cancer treatment.

MeSH terms

  • ADAM Proteins / chemistry
  • ADAM Proteins / genetics*
  • Aged
  • Aged, 80 and over
  • Animals
  • Base Sequence
  • Binding Sites
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation
  • Colorectal Neoplasms / genetics*
  • Colorectal Neoplasms / pathology
  • Disease Models, Animal
  • Down-Regulation
  • Female
  • Gene Expression
  • Gene Expression Regulation, Neoplastic
  • Genes, Reporter
  • Humans
  • Male
  • Mice
  • MicroRNAs / chemistry
  • MicroRNAs / genetics*
  • Middle Aged
  • Neoplasm Grading
  • Neoplasm Staging
  • RNA Interference*
  • Transfection
  • Tumor Burden
  • Xenograft Model Antitumor Assays

Substances

  • MIRN30b microRNA, human
  • MicroRNAs
  • ADAM Proteins
  • ADAM19 protein, human

Grants and funding

The authors have no support or funding to report.