Abstract
The discovery, synthesis, and characterization of 9H-carbazole-1-carboxamides as potent and selective ATP-competitive inhibitors of Janus kinase 2 (JAK2) are discussed. Optimization for JAK family selectivity led to compounds 14 and 21, with greater than 45-fold selectivity for JAK2 over all other members of the JAK kinase family.
Keywords:
JAK; JAK family selectivity; JAK1; JAK2; JAK3; Myeloproliferative disorders; Myeloproliferative neoplasms; TYK2.
Copyright © 2015 Elsevier Ltd. All rights reserved.
MeSH terms
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Amides / chemistry*
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Amides / metabolism
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Amides / pharmacology
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Binding Sites
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Carbazoles / chemistry
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Crystallography, X-Ray
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Humans
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Janus Kinase 1 / antagonists & inhibitors
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Janus Kinase 1 / metabolism
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Janus Kinase 2 / antagonists & inhibitors*
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Janus Kinase 2 / metabolism
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Janus Kinase 3 / antagonists & inhibitors
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Janus Kinase 3 / metabolism
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Molecular Dynamics Simulation
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Protein Binding
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Protein Kinase Inhibitors / chemistry*
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Protein Kinase Inhibitors / metabolism
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Protein Kinase Inhibitors / pharmacology
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Protein Structure, Tertiary
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Structure-Activity Relationship
Substances
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Amides
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Carbazoles
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Protein Kinase Inhibitors
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carbazole
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JAK2 protein, human
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Janus Kinase 1
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Janus Kinase 2
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Janus Kinase 3