A water-soluble glucan, BCG-PASW, with a molecular weight of 2.10×10(4)Da, was separated from polysaccharide nucleic acid fraction of Bacillus Calmette Guerin (BCG-PSN) using DEAE-52 cellulose and Sephadex G-200 chromatography. Based on gas chromatography-mass spectrometry (GC-MS), fourier transform infrared (FT-IR) spectra, 1D and 2D nuclear magnetic resonance (NMR) spectroscopy techniques (COSY, HSQC and HMBC), BCG-PASW was found to be an α-d-glucan composed of α-d-(1→4)-linked glucopyranosyl residues, with branches at O-6 consisting of non-reducing terminal α-d-Glcp approximately every eight residues. In vitro antitumor activity by MTS method, its sulfated derivative with a substitution degree of 0.59, could inhibite C666-1 nasopharyngeal carcinoma cells growth significantly. The results indicated that the sulfate content play a decisive role in the bioactivities of the polysaccharides.
Keywords: Antitumor; BCG-PSN; Characterization; Chlorosulfonic acid (CID: 24638); Methoxyamine (CID: 4113); N,O-Bis(trime.silyl)trifluoroacetamide (CID: 5366669); Purification; Sulfated derivative; d-Arabinose (CID: 66308); d-Glucopyranose (CID: 5793); d-Mannose (CID: 18950); d-Ribose (CID: 5311110).
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