Up in Arms: Immune and Nervous System Response to Sea Star Wasting Disease

PLoS One. 2015 Jul 15;10(7):e0133053. doi: 10.1371/journal.pone.0133053. eCollection 2015.

Abstract

Echinoderms, positioned taxonomically at the base of deuterostomes, provide an important system for the study of the evolution of the immune system. However, there is little known about the cellular components and genes associated with echinoderm immunity. The 2013-2014 sea star wasting disease outbreak is an emergent, rapidly spreading disease, which has led to large population declines of asteroids in the North American Pacific. While evidence suggests that the signs of this disease, twisting arms and lesions, may be attributed to a viral infection, the host response to infection is still poorly understood. In order to examine transcriptional responses of the sea star Pycnopodia helianthoides to sea star wasting disease, we injected a viral sized fraction (0.2 μm) homogenate prepared from symptomatic P. helianthoides into apparently healthy stars. Nine days following injection, when all stars were displaying signs of the disease, specimens were sacrificed and coelomocytes were extracted for RNA-seq analyses. A number of immune genes, including those involved in Toll signaling pathways, complement cascade, melanization response, and arachidonic acid metabolism, were differentially expressed. Furthermore, genes involved in nervous system processes and tissue remodeling were also differentially expressed, pointing to transcriptional changes underlying the signs of sea star wasting disease. The genomic resources presented here not only increase understanding of host response to sea star wasting disease, but also provide greater insight into the mechanisms underlying immune function in echinoderms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Complement System Proteins / genetics
  • Complement System Proteins / immunology
  • Densovirus / pathogenicity
  • Densovirus / physiology
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Immune System / metabolism*
  • Immune System / virology
  • Molecular Sequence Annotation
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / immunology
  • Nervous System / immunology
  • Nervous System / metabolism*
  • Nervous System / virology
  • Pacific Ocean
  • Signal Transduction
  • Starfish / virology*
  • Toll-Like Receptors / genetics
  • Toll-Like Receptors / immunology
  • Wasting Syndrome / immunology*
  • Wasting Syndrome / pathology
  • Wasting Syndrome / veterinary*
  • Wasting Syndrome / virology

Substances

  • Nerve Tissue Proteins
  • Toll-Like Receptors
  • Complement System Proteins

Grants and funding

This work was completed as part of the Ecology of Infectious Marine Diseases Research Coordination Network Workshop, funded by the National Science Foundation (OCE #1215977; http://www.eeb.cornell.edu/ecologymarinedisease/Home/Home.html; http://www.nsf.gov/funding/pgm_summ.jsp?pims_id=11691&org=OCE). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.