Molecular profile of sensitization in subjects with short occupational exposure to latex

Int J Occup Med Environ Health. 2015;28(5):841-8. doi: 10.13075/ijomeh.1896.00413.

Abstract

Objectives: We examined the prevalence of latex allergy in subjects with occupational exposure to latex allergens for less than 5 years, determining the disease spectrum in symptomatic workers. We identified the most frequent molecular allergens by Immuno- CAP (ICAP), correlating the findings with skin prick test (SPT) results.

Material and methods: Seven hundred twenty-three healthcare students using latex gloves on a regular basis were invited to participate in a baseline questionnaire screening. An ICAP serum test was performed only when a possible latex allergy was indicated by the questionnaire.

Results: The total number of participants responding to the baseline survey was 619. Glove-related symptoms were indicated by 4% (N = 25) of the students. The most common symptom was contact dermatitis (N = 18, 72%). In 12 subjects, ICAP revealed a real sensitization to latex, with a recombinant latex allergen profile showing a high frequency for rHev b 6.01 specific immunoglobulin E (sIgE) (N = 9, 67%). In these individuals, skin symptoms were more prevalent than other types (88%).

Conclusions: The combined positivity for rHev b 6.01, rHev 8 and rHev b 5 determined by ICAP identified 92% of latex-allergic subjects with short-term exposure to latex.

Keywords: ImmunoCAP; contact dermatitis; gloves; natural rubber latex; occupational exposure; skin prick test.

MeSH terms

  • Female
  • Gloves, Protective / adverse effects*
  • Health Personnel*
  • Humans
  • Italy / epidemiology
  • Latex / adverse effects
  • Latex / analysis*
  • Latex Hypersensitivity / chemically induced
  • Latex Hypersensitivity / epidemiology*
  • Male
  • Occupational Diseases / chemically induced
  • Occupational Diseases / epidemiology*
  • Occupational Exposure / adverse effects
  • Occupational Exposure / analysis*
  • Prevalence
  • Young Adult

Substances

  • Latex