MUCOSAL IMMUNOLOGY. Individual intestinal symbionts induce a distinct population of RORγ⁺ regulatory T cells

Science. 2015 Aug 28;349(6251):993-7. doi: 10.1126/science.aaa9420. Epub 2015 Aug 13.

Abstract

T regulatory cells that express the transcription factor Foxp3 (Foxp3(+) T(regs)) promote tissue homeostasis in several settings. We now report that symbiotic members of the human gut microbiota induce a distinct T(reg) population in the mouse colon, which constrains immuno-inflammatory responses. This induction—which we find to map to a broad, but specific, array of individual bacterial species—requires the transcription factor Rorγ, paradoxically, in that Rorγ is thought to antagonize FoxP3 and to promote T helper 17 (T(H)17) cell differentiation. Rorγ's transcriptional footprint differs in colonic T(regs) and T(H)17 cells and controls important effector molecules. Rorγ, and the T(regs) that express it, contribute substantially to regulating colonic T(H)1/T(H)17 inflammation. Thus, the marked context-specificity of Rorγ results in very different outcomes even in closely related cell types.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteria / immunology
  • Bacteroidetes / immunology
  • Bacteroidetes / physiology
  • Colitis, Ulcerative / immunology
  • Colon / immunology*
  • Colon / microbiology
  • Forkhead Transcription Factors / analysis
  • Forkhead Transcription Factors / metabolism
  • Homeostasis
  • Humans
  • Immunity, Mucosal*
  • Intestinal Mucosa / immunology*
  • Intestinal Mucosa / microbiology
  • Mice, Inbred C57BL
  • Microbiota / immunology*
  • Microbiota / physiology
  • Nuclear Receptor Subfamily 1, Group F, Member 3 / genetics
  • Nuclear Receptor Subfamily 1, Group F, Member 3 / metabolism*
  • Symbiosis
  • T-Lymphocyte Subsets / immunology
  • T-Lymphocytes, Regulatory / immunology*
  • Th17 Cells / immunology
  • Transcription, Genetic
  • Transcriptome

Substances

  • Forkhead Transcription Factors
  • Foxp3 protein, mouse
  • Nuclear Receptor Subfamily 1, Group F, Member 3
  • RORC protein, human
  • Rorc protein, mouse

Associated data

  • GEO/GSE68009