[Analysis of point mutations of Ha-ras gene in basaliomas and spinaliomas usiang the polymerase chain reaction (PCR) technic]

G Ital Dermatol Venereol. 1989 Oct;124(10):429-34.
[Article in Italian]

Abstract

Polymerase chain reaction is a recently developed procedure to detect point mutations in human genomic DNA. The sensitivity and specificity of this technique which requires a very little amount of DNA, have allowed the large scale screening of tumors. The Authors report the detection of point mutations of Ha-ras gene at codon 12 in 12 basal cell carcinomas and 10 cutaneous squamous cell carcinomas by the use of polymerase chain reaction in paraffin-embedded tissue sections. The gene alteration was identified in 2 basaliomas whereas no squamous cell carcinoma showed point mutations. These results suggest that point mutations of Ha-ras gene do not represent a general activating mechanism in cutaneous epitheliomas.

Publication types

  • English Abstract

MeSH terms

  • Carcinoma, Basal Cell / genetics*
  • Carcinoma, Squamous Cell / genetics*
  • Codon
  • Humans
  • Mutation*
  • Polymerase Chain Reaction
  • Skin Neoplasms / genetics*

Substances

  • Codon