Effects of Gastrodiae rhizoma on proliferation and differentiation of human embryonic neural stem cells

Samrat Baral; Ramesh Pariyar; Chi-Su Yoon; Dong-Cheol Kim; Jong-Min Yun; Seok O Jang; Sung Yeon Kim; Hyuncheol Oh; Youn-Chul Kim; Jungwon Seo

doi:10.1016/j.apjtm.2015.09.004

Effects of Gastrodiae rhizoma on proliferation and differentiation of human embryonic neural stem cells

Asian Pac J Trop Med. 2015 Oct;8(10):792-7. doi: 10.1016/j.apjtm.2015.09.004. Epub 2015 Sep 25.

Authors

Samrat Baral¹, Ramesh Pariyar², Chi-Su Yoon³, Dong-Cheol Kim³, Jong-Min Yun⁴, Seok O Jang⁴, Sung Yeon Kim⁵, Hyuncheol Oh⁶, Youn-Chul Kim⁷, Jungwon Seo⁸

Affiliations

¹ From the BK21 Plus Team, South Korea; Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 570-749, South Korea; Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan 570-749, South Korea; Hanbang Body-Fluid Research Center, Wonkwang University, Iksan 570-749, South Korea.
² Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 570-749, South Korea; Hanbang Body-Fluid Research Center, Wonkwang University, Iksan 570-749, South Korea.
³ Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 570-749, South Korea; Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University, Iksan 570-749, South Korea.
⁴ Department of Internal Medicine, College of Korean Medicine, Wonkwang University, Iksan 570-749, South Korea.
⁵ Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 570-749, South Korea.
⁶ Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 570-749, South Korea; Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan 570-749, South Korea; Hanbang Body-Fluid Research Center, Wonkwang University, Iksan 570-749, South Korea; Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University, Iksan 570-749, South Korea.
⁷ From the BK21 Plus Team, South Korea; Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 570-749, South Korea; Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan 570-749, South Korea; Hanbang Body-Fluid Research Center, Wonkwang University, Iksan 570-749, South Korea; Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University, Iksan 570-749, South Korea.
⁸ From the BK21 Plus Team, South Korea; Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 570-749, South Korea; Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan 570-749, South Korea; Hanbang Body-Fluid Research Center, Wonkwang University, Iksan 570-749, South Korea. Electronic address: [email protected].

PMID: 26522293
DOI: 10.1016/j.apjtm.2015.09.004

Abstract

Objective: To investigate the effects of Gastrodiae rhizoma, a dried root of Gastrodia elata Blume, on proliferation and differentiation of human NSCs derived from embryonic stem cells.

Methods: A 70% ethanol extract of Gastrodiae rhizoma (EEGR) was estimated with 4-hydroxybenzyl alcohol as a representative constituent by HPLC.

Results: MTT assay showed that the treatment with EEGR increased the viability of NSCs in growth media. Compared to control, EEGR increased the number of dendrites and denritic spines extended from a differentiated NSC. Whereas EEGR decreased the mRNA expression of Nestin, it increased that of Tuj1 and MAP2 in NSCs grown in differentiation media. Immunocytochemical analysis using confocal microscopy also revealed the increased expression of MAP2 in dendrites of EEGR-treated NSCs. Furthermore, EEGR decreased mRNA expression of Sox2 in NSCs grown even in growth media.

Conclusions: In conclusion, our study demonstrates for the first time that EEGR induced proliferation and neuronal differentiation of NSCs, suggesting its potential benefits on NSC-based therapies and neuroregeneration in various neurodegenerative diseases and brain injuries.

Keywords: Dendrite; Gastrodiae rhizoma; MAP2; Neural stem cells; Neuronal differentiation; Proliferation.