Biowire platform for maturation of human pluripotent stem cell-derived cardiomyocytes

Xuetao Sun; Sara S Nunes

doi:10.1016/j.ymeth.2015.11.005

Biowire platform for maturation of human pluripotent stem cell-derived cardiomyocytes

Methods. 2016 May 15:101:21-6. doi: 10.1016/j.ymeth.2015.11.005. Epub 2015 Nov 4.

Authors

Xuetao Sun¹, Sara S Nunes²

Affiliations

¹ University Health Network, Toronto General Research Institute, 101 College St., Canada.
² University Health Network, Toronto General Research Institute, 101 College St., Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Canada; Heart & Stroke/Richard Lewar Centre of Excellence, University of Toronto, Canada. Electronic address: [email protected].

PMID: 26546730
DOI: 10.1016/j.ymeth.2015.11.005

Abstract

Human pluripotent stem cells (hPSCs)-derived cardiomyocytes (hPSC-CMs) represent a potential indefinite cell supply for cardiac tissue engineering and possibly regenerative medicine applications. However, these cells are immature compared with adult ventricular cardiomyocytes. In order to overcome this limitation, an engineered platform, called biowire, was devised to provide cultured cardiomyocytes important biomimetic cues present during embryo development, such as three-dimensional cell culture, extracellular matrix composition, soluble factors and pacing through electrical stimulation, to induce the maturation of hPSC-CMs in vitro.

Keywords: Biomimetic cues; Cardiomyocyte; Maturation; Stem cells; Tissue engineering.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques*
Cell Differentiation
Electric Stimulation
Humans
Myocytes, Cardiac / physiology*
Pluripotent Stem Cells / physiology*

Grants and funding

137352/CIHR/Canada