Identification of hub genes of pneumocyte senescence induced by thoracic irradiation using weighted gene co‑expression network analysis

Mol Med Rep. 2016 Jan;13(1):107-16. doi: 10.3892/mmr.2015.4566. Epub 2015 Nov 13.

Abstract

Irradiation commonly causes pneumocyte senescence, which may lead to severe fatal lung injury characterized by pulmonary dysfunction and respiratory failure. However, the molecular mechanism underlying the induction of pneumocyte senescence by irradiation remains to be elucidated. In the present study, weighted gene co‑expression network analysis (WGCNA) was used to screen for differentially expressed genes, and to identify the hub genes and gene modules, which may be critical for senescence. A total of 2,916 differentially expressed genes were identified between the senescence and non‑senescence groups following thoracic irradiation. In total, 10 gene modules associated with cell senescence were detected, and six hub genes were identified, including B‑cell scaffold protein with ankyrin repeats 1, translocase of outer mitochondrial membrane 70 homolog A, actin filament‑associated protein 1, Cd84, Nuf2 and nuclear factor erythroid 2. These genes were markedly associated with cell proliferation, cell division and cell cycle arrest. The results of the present study demonstrated that WGCNA of microarray data may provide further insight into the molecular mechanism underlying pneumocyte senescence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alveolar Epithelial Cells / cytology*
  • Alveolar Epithelial Cells / metabolism*
  • Animals
  • Cellular Senescence / genetics*
  • Cellular Senescence / radiation effects*
  • Gene Expression Profiling
  • Gene Expression Regulation / radiation effects*
  • Gene Ontology
  • Gene Regulatory Networks / radiation effects*
  • Genetic Association Studies
  • Mice
  • Molecular Sequence Annotation
  • Signal Transduction / genetics
  • Signal Transduction / radiation effects
  • Thorax / radiation effects*