Gut-Resident Lactobacillus Abundance Associates with IDO1 Inhibition and Th17 Dynamics in SIV-Infected Macaques

Cell Rep. 2015 Nov 24;13(8):1589-97. doi: 10.1016/j.celrep.2015.10.026. Epub 2015 Nov 12.

Abstract

Gut microbes can profoundly modulate mucosal barrier-promoting Th17 cells in mammals. A salient feature of HIV/simian immunodeficiency virus (SIV) immunopathogenesis is the loss of Th17 cells, which has been linked to increased activity of the immunomodulatory enzyme, indoleamine 2,3-dioxygenase 1 (IDO 1). The role of gut microbes in this system remains unknown, and the SIV-infected rhesus macaque provides a well-described model for HIV-associated Th17 loss and mucosal immune disruption. We observed a specific depletion of gut-resident Lactobacillus during acute and chronic SIV infection of rhesus macaques, which was also seen in early HIV-infected humans. This depletion in rhesus macaques correlated with increased IDO1 activity and Th17 loss. Macaques supplemented with a Lactobacillus-containing probiotic exhibited decreased IDO1 activity during chronic SIV infection. We propose that Lactobacillus species inhibit mammalian IDO1 and thus may help to preserve Th17 cells during pathogenic SIV infection, providing support for Lactobacillus species as modulators of mucosal immune homeostasis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Female
  • HIV Infections / immunology
  • HIV Infections / microbiology
  • Indoleamine-Pyrrole 2,3,-Dioxygenase / immunology*
  • Intestinal Mucosa / immunology*
  • Intestinal Mucosa / microbiology*
  • Lactobacillus / immunology*
  • Macaca mulatta / immunology*
  • Simian Acquired Immunodeficiency Syndrome / immunology
  • Simian Acquired Immunodeficiency Syndrome / microbiology
  • Simian Immunodeficiency Virus / immunology*
  • Th17 Cells / immunology*
  • Th17 Cells / microbiology

Substances

  • Indoleamine-Pyrrole 2,3,-Dioxygenase