The bone marrow represents an enrichment site of specific T lymphocytes against filamentous fungi

Med Mycol. 2016 Mar;54(3):327-32. doi: 10.1093/mmy/myv107. Epub 2015 Dec 24.

Abstract

Bone marrow has already been described as an enrichment site for several antigen-specific T lymphocytes, but the presence of mould-specific T cells has never been investigated in the bone marrow. We have previously demonstrated that mould-specific T cells emerge in the peripheral blood of patients with invasive fungal infections (IFI) but tend to become undetectable after disease resolution. In seven patients with a history of IFI, we investigated the presence of mould-specific T cells secreting different cytokines in bone marrow and peripheral blood paired samples. The results showed that the frequencies of mould-specific T cells secreting the protective cytokine IFNγ are significantly higher in bone marrow (BM) and are mainly represented by CD8+ T lymphocytes with effector phenotype. A putative disappearance of such protective BM responses after myeloablative therapy could contribute to the increased risk of IFI in hematologic patients.

Keywords: Invasive Aspergillosis; Invasive Mucormycosis; antigen-specific T cells; bone marrow.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Blood / immunology
  • Bone Marrow / immunology*
  • CD8-Positive T-Lymphocytes / immunology*
  • Cohort Studies
  • Female
  • Fungemia / immunology*
  • Fungi / immunology*
  • Humans
  • Interferon-gamma / metabolism
  • Male
  • Middle Aged

Substances

  • Interferon-gamma