Bone tissue damages are one of the dominant causes of temporary disability and developmental disability. Currently, there are some methods of guided bone regeneration employing different osteoplastic materials and insulation membranes used in surgery. In this study, we have developed a method of preparation of porous membranes from the biopolymer poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV), produced by a strain of Azotobacter chroococcum 7B. The biocompatibility of the porous membranes was investigated in vitro using mesenchymal stem cells (MSCs) and in vivo on laboratory animals. The cytotoxicity test showed the possibility of cell attachment on membrane and histological studies confirmed good insulating properties the material. The data obtained demonstrate the high biocompatibility and the potential application of insulating membranes based on PHBV in bone tissue engineering.
Porazheniia kostnykh tkaneĭ zanimaiut odno iz pervykh mest sredi prichin vremennoĭ netrudosposobnosti i razvitiia invalidnosti. V nastoiashchee vremia v khirurgicheskoĭ praktike primeniaiutsia metodiki napravlennoĭ kostnoĭ regeneratsii s ispol'zovaniem razlichnykh osteoplasticheskikh materialov i izoliruiushchikh membran. Nami razrabotana metodika polucheniia poristykh membran iz sopolimera poli-3-oksibutirata s 3-oksivaleratom (POBV), produtsiruemogo shtammom Azotobacter chroococcum 7B, i provedeny issledovaniia dannoĭ podlozhki na biosovmestimost' kak na kletochnoĭ kul'ture mezenkhimal'nykh stvolovykh kletok (MSK), tak i na laboratornykh zhivotnykh. Rezul'taty testa na tsitotoksichnost' i gistologicheskikh issledovaniĭ svidetel'stvuiut o vysokoĭ biosovmesimosti dannogo materiala i potentsial'noĭ vozmozhnosti ispol'zovaniia ego v tkanevoĭ inzhenerii kostnoĭ tkani.
Keywords: bone tissue; guided bone regeneration; isolating membrane; poly(3-hydroxybutyrate-co-3-hydroxyvalerate); porous scaffold.