Objective: To observe the short- term effects of hemogram in donors after peripheral blood stem cell（PBSC）collection and donors' tolerance.
Methods: A total of 166 related allogeneic donors were selected from The First Affiliated Hospital of Medical School of Zhejiang University between January 2013 and December 2014, including 86 male and 80 female. All donors accepted granulocytecolony- stimulating factor（G-CSF）5-10 μg·kg⁻¹·d⁻¹ until collection finished and were measured by blood cells count before and after PBSC collection.
Results: After PBSC collection, the hemoglobin level decreased from 145（94-181）g/L to 138（93-167）g/L, and the platelet counts decreased in all donors from 231（105- 490）× 10⁹/L to 95（39- 210）× 10⁹/L. The amount of hemoglobin contamination in collection products was weak correlated with the decreased hemoglobin in peripheral blood（r=0.297, P=0.017）, and the platelet contamination was high correlated with that decreased in peripheral blood（r=0.719, P<0.001）. The decline of hemoglobin level after twice PBSC collection was of no significant difference between four groups in different ages（P≥0.05）. The decline of platelet counts was out of a significant difference（P> 0.05）. In addition, the decline of hemoglobin level after once and twice PBSC collection was of a significant difference between four groups in different body mass index（BMI）（P=0.003 and P<0.001）, especially in thinner group with obvious decrease. But the decline of platelet counts was out of a significant difference （P>0.05）.
Conclusion: The hemoglobin level decreased mildly in healthy allogeneic hematopoietic stem cell donors after PBSC collection and it is better to adjust parameters every time to ensure their safety for thinner donors. However, it will increase the risk of platelet decline, which is unrelated with ages and BMI and can be tolerated.
目的: 探讨连续外周血造血干细胞（PBSC）采集对异基因造血干细胞供者外周血细胞计数的影响以及供者对连续采集的耐受性。
方法: 166名于2013至2014年在浙江大学医学院附属第一医院血液科捐献PBSC的亲缘异基因供者，男86名，女80名，中位年龄40.5（15.0~60.0）岁。动员方案为G-CSF 5~10 µg·kg−1·d−1皮下注射至采集结束。于PBSC动员前、采集前、采集1次及2次后检测供者外周血以及采集物中WBC、PLT、HGB。
结果: 连续2次PBSC采集后供者外周血HGB中位数由145（94~181）g/L降至138（93~167）g/L（P<0.05），PLT中位数由231（105~490）×109/L降至95（39~210）× 109/L（P<0.05），WBC差异无统计学意义（P>0.05）。采集物中HGB与供者外周血HGB降幅呈低度正相关（r=0.297，P=0.017），采集物中PLT与供者外周血PLT降幅高度相关（r=0.719，P<0.001）。不同年龄组供者在PBSC采集后的HGB降幅两两比较差异无统计学意义（P≥0.05）。PLT差异无统计学意义（P>0.05）。不同体重指数供者在PBSC采集1次及2次后外周血HGB降幅差异均有统计学意义（P=0.003，P<0.001），体重指数<18.5 kg/m2的偏瘦组供者HGB下降最明显（P<0.05）。
结论: 健康异基因造血干细胞供者在连续PBSC采集过程中HGB轻度下降，对于偏瘦供者应调整采集参数以保证安全。采集PBSC会使血小板减少的风险增加，减少的程度与年龄及体重指数无关，且均可耐受。