Sensing Conductive Hydrogels for Rapid Detection of Cytokines in Blood

Adv Healthc Mater. 2016 Mar;5(6):659-64, 627. doi: 10.1002/adhm.201500571. Epub 2016 Jan 22.

Abstract

Conducting polymer hydrogel is fabricated atop gold or ITO electrodes and is functionalized with monoclonal antibodies. Binding of interferon-γ molecules causes redox properties of conductive hydrogel to change in a concentration-dependent fashion without the need for washing or sample handling steps. This conductive hydrogel remains functional in a fouling media such as whole blood.

Keywords: biosensors; conducting polymer hydrogels; cytokine detection; electrochemical detection; immunoassays.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biosensing Techniques
  • Bridged Bicyclo Compounds, Heterocyclic / chemistry
  • Cattle
  • Cytokines / blood*
  • Electrochemical Techniques*
  • Electrodes
  • Gold / chemistry
  • Hydrogels / chemistry*
  • Interferon-gamma / blood
  • Leukocytes, Mononuclear / cytology
  • Leukocytes, Mononuclear / metabolism
  • Polymers / chemistry
  • Tin Compounds / chemistry

Substances

  • Bridged Bicyclo Compounds, Heterocyclic
  • Cytokines
  • Hydrogels
  • Polymers
  • Tin Compounds
  • poly(3,4-ethylene dioxythiophene)
  • indium tin oxide
  • Gold
  • Interferon-gamma