New 4-Functionalized Glutamate Analogues Are Selective Agonists at Metabotropic Glutamate Receptor Subtype 2 or Selective Agonists at Metabotropic Glutamate Receptor Group III

J Med Chem. 2016 Feb 11;59(3):914-24. doi: 10.1021/acs.jmedchem.5b01333. Epub 2016 Jan 27.

Abstract

The metabotropic glutamate (Glu) receptors (mGluRs) play key roles in modulating excitatory neurotransmission in the brain. In all, eight subtypes have been identified and divided into three groups, group I (mGlu1,5), group II (mGlu2,3), and group III (mGlu4,6-8). In this article, we present a L-2,4-syn-substituted Glu analogue, 1d, which displays selective agonist activity at mGlu2 over the remaining mGluR subtypes. A modeling study and redesign of the core scaffold led to the stereoselective synthesis of four new conformationally restricted Glu analogues, 2a-d. Most interestingly, 2a retained a selective agonist activity profile at mGlu2 (EC50 in the micromolar range), whereas 2c/2d were both selective agonists at group III, subtypes mGlu4,6,8. In general, 2d was 20-fold more potent than 2c and potently activated mGlu4,6,8 in the low-mid nanomolar range.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Crystallography, X-Ray
  • Dose-Response Relationship, Drug
  • Glutamic Acid / analogs & derivatives*
  • Glutamic Acid / chemical synthesis
  • Glutamic Acid / chemistry
  • Glutamic Acid / pharmacology*
  • HEK293 Cells
  • Humans
  • Models, Molecular
  • Molecular Structure
  • Rats
  • Receptors, Metabotropic Glutamate / agonists*
  • Structure-Activity Relationship

Substances

  • Receptors, Metabotropic Glutamate
  • metabotropic glutamate receptor 2
  • metabotropic glutamate receptor 3
  • Glutamic Acid