A Mitochondrion-Specific Photoactivatable Fluorescence Turn-On AIE-Based Bioprobe for Localization Super-Resolution Microscope

Xinggui Gu; Engui Zhao; Teng Zhao; Miaomiao Kang; Chen Gui; Jacky W Y Lam; Shengwang Du; Michael M T Loy; Ben Zhong Tang

doi:10.1002/adma.201505906

A Mitochondrion-Specific Photoactivatable Fluorescence Turn-On AIE-Based Bioprobe for Localization Super-Resolution Microscope

Adv Mater. 2016 Jul;28(25):5064-71. doi: 10.1002/adma.201505906. Epub 2016 May 2.

Authors

Xinggui Gu^{1

2}, Engui Zhao^{1

2}, Teng Zhao², Miaomiao Kang^{2

3}, Chen Gui^{1

2}, Jacky W Y Lam^{1

2}, Shengwang Du², Michael M T Loy², Ben Zhong Tang^{1

2

4}

Affiliations

¹ HKUST-Shenzhen Research Institute, No. 9 Yuexing 1st RD, South Area, Hi-tech Park Nanshan, Shenzhen, 518057, China.
² Departments of Chemistry and Physics, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Division of Life Science, Institute of Molecular Functional Materials and Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
³ Department of Neurobiology and Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.
⁴ Guangdong Innovative Research Team, SCUT-HKUST Joint Research Laboratory, State Key Laboratory of Luminescent Materials and Device, South China University of Technology, Guangzhou, 51640, China.

PMID: 27135807
DOI: 10.1002/adma.201505906

Abstract

A novel mitochondrion-specific photo-activatable fluorescence turn-on bioprobe, named as o-TPE-ON+, is designed and readily prepared, operating through a new photoactivatable mechanism of photocyclodehydrogenation. This bioprobe exhibits unique photoactivation behavior in cells, and is applied to super-resolution imaging of mitochondrion and its dynamic investigation in both fixed and live cells under physiological conditions without any external additives.

Keywords: fluorescence turn-on; mitochondrion-specific; photoactivatable fluorophores; photocyclodehydrogenation; super-resolution imaging.

MeSH terms

Biosensing Techniques
Fluorescent Dyes
Microscopy, Fluorescence
Mitochondria*

Substances

Fluorescent Dyes