PCR-Based Serotyping of Streptococcus pneumoniae from Culture-Negative Specimens: Novel Primers for Detection of Serotypes within Serogroup 18

Arif M Tanmoy; Senjuti Saha; Gary L Darmstadt; Cynthia G Whitney; Samir K Saha

doi:10.1128/JCM.00419-16

PCR-Based Serotyping of Streptococcus pneumoniae from Culture-Negative Specimens: Novel Primers for Detection of Serotypes within Serogroup 18

J Clin Microbiol. 2016 Aug;54(8):2178-81. doi: 10.1128/JCM.00419-16. Epub 2016 Jun 1.

Authors

Arif M Tanmoy¹, Senjuti Saha², Gary L Darmstadt³, Cynthia G Whitney⁴, Samir K Saha⁵

Affiliations

¹ Child Health Research Foundation, Department of Microbiology, Dhaka Shishu Hospital, Dhaka, Bangladesh.
² Department of Molecular Genetics, University of Toronto, Toronto, Canada.
³ Department of Pediatrics, Stanford University School of Medicine, Stanford, California, USA.
⁴ Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
⁵ Child Health Research Foundation, Department of Microbiology, Dhaka Shishu Hospital, Dhaka, Bangladesh Bangladesh Institute of Child Health, Department of Microbiology, Dhaka Shishu Hospital, Dhaka, Bangladesh [email protected].

Abstract

Six multiplex-compatible PCR primers were designed to distinguish Streptococcus pneumoniae serotypes within serogroup 18 from culturable/nonculturable pneumococcal specimens, with no cross-reactivity with other serotypes and respiratory organisms. These primers will aid in the generation of better data on vaccine/nonvaccine serotypes in invasive and carriage pneumococcal surveillance and contribute to future vaccine formulation and impact studies.

MeSH terms

DNA Primers / genetics
Genotyping Techniques / methods*
Humans
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Serogroup
Serotyping / methods*
Streptococcal Infections / microbiology*
Streptococcus pneumoniae / classification*
Streptococcus pneumoniae / genetics
Streptococcus pneumoniae / isolation & purification

Substances

DNA Primers

Grants and funding

This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.