Generation of human control iPS cell line CHOPWT10 from healthy adult peripheral blood mononuclear cells

Jean Ann Maguire; Alyssa L Gagne; Chintan D Jobaliya; Shilpa Gandre-Babbe; Paul Gadue; Deborah L French

doi:10.1016/j.scr.2016.02.017

Generation of human control iPS cell line CHOPWT10 from healthy adult peripheral blood mononuclear cells

Stem Cell Res. 2016 Mar;16(2):338-41. doi: 10.1016/j.scr.2016.02.017. Epub 2016 Feb 3.

Authors

Jean Ann Maguire¹, Alyssa L Gagne¹, Chintan D Jobaliya¹, Shilpa Gandre-Babbe², Paul Gadue¹, Deborah L French¹

Affiliations

¹ Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, United States; Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, University of Pennsylvania, United States.
² Coriell Institute for Medical Research, United States.

PMID: 27345999
DOI: 10.1016/j.scr.2016.02.017

Abstract

The CHOPWT10 iPS cell line was generated to be used as a control for applications such as in differentiation analyses to the three germ layers and derivative tissues. Peripheral blood mononuclear cells (PBMCs) obtained from a healthy adult male were reprogrammed using the non-integrating Sendai virus expressing Oct3/4, Sox2, c-Myc, and Klf4.

MeSH terms

Adult
Cell Differentiation
Cells, Cultured
Cellular Reprogramming
Embryoid Bodies / cytology
Flow Cytometry
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism
Karyotype
Kruppel-Like Factor 4
Leukocytes, Mononuclear / cytology*
Male
Microscopy, Fluorescence
Real-Time Polymerase Chain Reaction
Transcription Factors / genetics
Transcription Factors / metabolism

Substances

KLF4 protein, human
Kruppel-Like Factor 4
Transcription Factors