Elevated Amygdala Perfusion Mediates Developmental Sex Differences in Trait Anxiety

Biol Psychiatry. 2016 Nov 15;80(10):775-785. doi: 10.1016/j.biopsych.2016.04.021. Epub 2016 May 9.

Abstract

Background: Adolescence is a critical period for emotional maturation and is a time when clinically significant symptoms of anxiety and depression increase, particularly in females. However, few studies relate developmental differences in symptoms of anxiety and depression to brain development. Cerebral blood flow is one brain phenotype that is known to have marked developmental sex differences.

Methods: We investigated whether developmental sex differences in cerebral blood flow mediated sex differences in anxiety and depression symptoms by capitalizing on a large sample of 875 youths who completed cross-sectional imaging as part of the Philadelphia Neurodevelopmental Cohort. Perfusion was quantified on a voxelwise basis using arterial spin-labeled magnetic resonance imaging at 3T. Perfusion images were related to trait and state anxiety using general additive models with penalized splines, while controlling for gray matter density on a voxelwise basis. Clusters found to be related to anxiety were evaluated for interactions with age, sex, and puberty.

Results: Trait anxiety was associated with elevated perfusion in a network of regions including the amygdala, anterior insula, and fusiform cortex, even after accounting for prescan state anxiety. Notably, these relationships strengthened with age and the transition through puberty. Moreover, higher trait anxiety in postpubertal females was mediated by elevated perfusion of the left amygdala.

Conclusions: Taken together, these results demonstrate that differences in the evolution of cerebral perfusion during adolescence may be a critical element of the affective neurobiological characteristics underlying sex differences in anxiety and mood symptoms.

Keywords: Adolescence; Amygdala; Anxiety; Cerebral blood flow; Depression; Insula; Perfusion.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adolescent Development / physiology*
  • Adult
  • Amygdala / physiology*
  • Amygdala / physiopathology
  • Anxiety* / physiopathology
  • Cerebral Cortex / physiology*
  • Cerebral Cortex / physiopathology
  • Cerebrovascular Circulation / physiology*
  • Child
  • Cross-Sectional Studies
  • Female
  • Humans
  • Magnetic Resonance Imaging / methods*
  • Male
  • Personality / physiology*
  • Sex Characteristics*
  • Spin Labels
  • Young Adult

Substances

  • Spin Labels