LncRNA CCAT2 predicts poor prognosis and regulates growth and metastasis in small cell lung cancer

Biomed Pharmacother. 2016 Aug:82:583-8. doi: 10.1016/j.biopha.2016.05.017. Epub 2016 Jun 7.

Abstract

LncRNA colon cancer-associated transcript 2 (CCAT2) was firstly discovered and found overexpressed in colorectal cancer, and identified as is oncogenic lncRNA. However, the significance of CCAT2 in small cell lung cancer (SCLC) remains unclear. The expression of CCAT2 in SCLC tissues and cell lines was detected, and the association between CCAT2 expression and clinicopathological factors was analyzed. Cell proliferation and invasion assays were conducted by using SCLC cell lines transfected with CCAT2-siRNA or NC-siRNA. In our results, CCAT2 expression was elevated in SCLC tissues and cell lines, and correlated with malignant status and poor prognosis of SCLC patients. Furthermore, knockdown of CCAT2 expression effectively suppressed SCLC cell growth and metastasis in vitro. In conclusion, CCAT2 serves as an oncogenic lncRNA, and an independent unfavorable prognostic factor in SCLC patients.

Keywords: CCAT2; LncRNA; Metastasis; Proliferation; SCLC.

MeSH terms

  • Cell Line, Tumor
  • Cell Proliferation
  • Disease Progression
  • Female
  • Gene Expression Regulation, Neoplastic
  • Gene Knockdown Techniques
  • Humans
  • Lung Neoplasms / genetics*
  • Lung Neoplasms / pathology*
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Neoplasm Metastasis
  • Prognosis
  • Proportional Hazards Models
  • RNA, Long Noncoding / genetics*
  • RNA, Long Noncoding / metabolism
  • Small Cell Lung Carcinoma / genetics*
  • Small Cell Lung Carcinoma / pathology*

Substances

  • RNA, Long Noncoding
  • long non-coding RNA CCAT2, human