Genetic Disruption of Arc/Arg3.1 in Mice Causes Alterations in Dopamine and Neurobehavioral Phenotypes Related to Schizophrenia

Cell Rep. 2016 Aug 23;16(8):2116-2128. doi: 10.1016/j.celrep.2016.07.044. Epub 2016 Aug 11.

Abstract

Human genetic studies have recently suggested that the postsynaptic activity-regulated cytoskeleton-associated protein (Arc) complex is a convergence signal for several genes implicated in schizophrenia. However, the functional significance of Arc in schizophrenia-related neurobehavioral phenotypes and brain circuits is unclear. Here, we find that, consistent with schizophrenia-related phenotypes, disruption of Arc in mice produces deficits in sensorimotor gating, cognitive functions, social behaviors, and amphetamine-induced psychomotor responses. Furthermore, genetic disruption of Arc leads to concomitant hypoactive mesocortical and hyperactive mesostriatal dopamine pathways. Application of a D1 agonist to the prefrontal cortex or a D2 antagonist in the ventral striatum rescues Arc-dependent cognitive or psychomotor abnormalities, respectively. Our findings demonstrate a role for Arc in the regulation of dopaminergic neurotransmission and related behaviors. The results also provide initial biological support implicating Arc in dopaminergic and behavioral abnormalities related to schizophrenia.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Intramural

MeSH terms

  • 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine / pharmacology
  • Amphetamine / pharmacology
  • Animals
  • Cognitive Dysfunction / genetics*
  • Cognitive Dysfunction / metabolism
  • Cognitive Dysfunction / physiopathology
  • Cognitive Dysfunction / prevention & control
  • Corpus Striatum / drug effects
  • Corpus Striatum / metabolism
  • Corpus Striatum / physiopathology
  • Cytoskeletal Proteins / deficiency
  • Cytoskeletal Proteins / genetics*
  • Disease Models, Animal
  • Dopamine / metabolism*
  • Dopamine Agonists / pharmacology
  • Dopamine Antagonists / pharmacology
  • Female
  • Gene Expression
  • Male
  • Mice
  • Mice, Knockout
  • Nerve Tissue Proteins / deficiency
  • Nerve Tissue Proteins / genetics*
  • Phenotype
  • Prefrontal Cortex / drug effects
  • Prefrontal Cortex / metabolism
  • Prefrontal Cortex / physiopathology
  • Psychomotor Disorders / genetics*
  • Psychomotor Disorders / metabolism
  • Psychomotor Disorders / physiopathology
  • Psychomotor Disorders / prevention & control
  • Receptors, Dopamine D1 / genetics
  • Receptors, Dopamine D1 / metabolism
  • Receptors, Dopamine D2 / genetics
  • Receptors, Dopamine D2 / metabolism
  • Salicylamides / pharmacology
  • Schizophrenia / genetics*
  • Schizophrenia / metabolism
  • Schizophrenia / physiopathology
  • Schizophrenia / prevention & control
  • Sensory Gating / drug effects
  • Sensory Gating / genetics
  • Synaptic Transmission

Substances

  • Cytoskeletal Proteins
  • Dopamine Agonists
  • Dopamine Antagonists
  • Nerve Tissue Proteins
  • Receptors, Dopamine D1
  • Receptors, Dopamine D2
  • Salicylamides
  • activity regulated cytoskeletal-associated protein
  • 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine
  • Amphetamine
  • eticlopride
  • Dopamine