Oncogenic Transformation of Human-Derived Gastric Organoids

Nina Bertaux-Skeirik; Jomaris Centeno; Jian Gao; Joel Gabre; Yana Zavros

doi:10.1007/7651_2016_4

Oncogenic Transformation of Human-Derived Gastric Organoids

Methods Mol Biol. 2019:1576:205-213. doi: 10.1007/7651_2016_4.

Authors

Nina Bertaux-Skeirik¹, Jomaris Centeno², Jian Gao³, Joel Gabre⁴, Yana Zavros⁵

Affiliations

¹ Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
² Biology Department, University of Puerto Rico-Río Piedras, San Juan, Puerto Rico.
³ Department of Pediatrics, W.F. Maternal & Child Health Hospital, 76 Qingnian Road, Weifang, 261011, People's Republic of China.
⁴ Department of Internal Medicine, University of Cincinnati, Cincinnati, OH, USA.
⁵ Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, 231 Albert B. Sabin Way, Room 4255 MSB, Cincinnati, OH, 45267-0576, USA. [email protected].

PMID: 27539460
DOI: 10.1007/7651_2016_4

Abstract

The culture of organoids has represented a significant advancement in the gastrointestinal research field. Previous research studies have described the oncogenic transformation of human intestinal and mouse gastric organoids. Here we detail the protocol for the oncogenic transformation and orthotopic transplantation of human-derived gastric organoids.

Keywords: CRISPR/Cas-9; Human fundic gastric organoids; Nucleofection; Organoid orthotopic transplantation.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Transformation, Neoplastic / pathology*
Heterografts
Humans
Mice
Organoids / pathology*
Organoids / transplantation*
Stomach Neoplasms / pathology*

Abstract

Publication types

MeSH terms

Grants and funding