GUIDE-Seq to Detect Genome-wide Double-Stranded Breaks in Plants

Trends Plant Sci. 2016 Oct;21(10):815-818. doi: 10.1016/j.tplants.2016.08.005. Epub 2016 Sep 10.

Abstract

Animal and plant cells have repair capabilities to combat DNA damage. DNA damage and repair dynamics can be determined by technologies such as IDLV capture, BLESS, HTGTS, digenome-seq, and GUIDE-seq. Here we highlight GUIDE-seq, a technology used in therapeutics, and envision its application in plants.

Keywords: BLESS; CRISPR/Cas9; DNA damage repair/response; GUIDE-seq; HTGTS; IDLV capture; PTO linkage; digenome-seq; double-stranded breaks; double-stranded oligonucleotide; single-stranded oligonucleotide.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • DNA Breaks, Double-Stranded*
  • DNA Breaks, Single-Stranded
  • DNA Damage
  • Genetic Techniques*
  • Genome, Plant / genetics
  • Oligonucleotides / genetics
  • Plants / genetics*
  • Sensitivity and Specificity

Substances

  • Oligonucleotides