A proposal for validation of antibodies

Mathias Uhlen; Anita Bandrowski; Steven Carr; Aled Edwards; Jan Ellenberg; Emma Lundberg; David L Rimm; Henry Rodriguez; Tara Hiltke; Michael Snyder; Tadashi Yamamoto

doi:10.1038/nmeth.3995

A proposal for validation of antibodies

Nat Methods. 2016 Oct;13(10):823-7. doi: 10.1038/nmeth.3995. Epub 2016 Sep 5.

Authors

Affiliations

¹ Science for Life Laboratory, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden.
² Center for Research in Biological Systems, University of California at San Diego, San Diego, California, USA.
³ Proteomics Platform, Broad Institute of Massachusetts Institute of Technology and Harvard University, Cambridge, Massachusetts, USA.
⁴ Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada.
⁵ Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
⁶ Department of Pathology, Yale University School of Medicine, New Haven, Connecticut, USA.
⁷ Office of Cancer Clinical Proteomics Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
⁸ Department of Genetics, Stanford University School of Medicine, Stanford, California, USA.
⁹ Biofluid Biomarker Center, Niigata University, Niigata, Japan.

Abstract

We convened an ad hoc International Working Group for Antibody Validation in order to formulate the best approaches for validating antibodies used in common research applications and to provide guidelines that ensure antibody reproducibility. We recommend five conceptual ‘pillars’ for antibody validation to be used in an application-specific manner.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies / immunology*
Antigens / immunology*
Humans
Reproducibility of Results
Validation Studies as Topic*

Substances

Antibodies
Antigens

Grants and funding

U24 DA039832/DA/NIDA NIH HHS/United States