Generation of human iPSCs from urine derived cells of a patient with a novel homozygous PAI-1 mutation

Stem Cell Res. 2016 Nov;17(3):657-660. doi: 10.1016/j.scr.2016.11.010. Epub 2016 Nov 9.

Abstract

We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subject with a novel homozygous Plasminogen Activator Inhibitor-1 (PAI-1 null) mutation. The Sendai virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Base Sequence
  • Cell Differentiation
  • Cell Line
  • Cellular Reprogramming
  • DNA Mutational Analysis
  • Embryoid Bodies / cytology
  • Embryoid Bodies / metabolism
  • Female
  • Genetic Vectors / genetics
  • Genetic Vectors / metabolism
  • Homozygote
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism
  • Karyotype
  • Microscopy, Fluorescence
  • Mutagenesis, Insertional
  • Plasminogen Activator Inhibitor 1 / genetics*
  • Sendai virus / genetics
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Urine / cytology

Substances

  • Plasminogen Activator Inhibitor 1
  • Transcription Factors