Abstract
Messenger RNA (mRNA) plays a critical role in cellular growth and development. However, there have been limited methods available to visualize endogenous mRNA in living cells with ease. We have designed RNA-based fluorescence "turn-on" probes that target mRNA by fusing an unstable form of Spinach with target-complementary sequences. These probes have been demonstrated to be selective, stable, and capable of targeting various mRNAs for live E. coli imaging.
MeSH terms
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Aptamers, Nucleotide / chemistry*
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Benzyl Compounds / chemistry*
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Escherichia coli / chemistry
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Escherichia coli / genetics
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Fluorescent Dyes / chemistry*
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G-Quadruplexes
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Imidazolines / chemistry*
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Nucleic Acid Hybridization
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RNA, Messenger / analysis*
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RNA, Messenger / chemistry
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Spectrometry, Fluorescence
Substances
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3,5-difluoro-4-hydroxybenzylidene imidazolinone
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Aptamers, Nucleotide
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Benzyl Compounds
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Fluorescent Dyes
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Imidazolines
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RNA, Messenger