Detection of Copy Number Alterations Using Single Cell Sequencing

J Vis Exp. 2017 Feb 17:(120):55143. doi: 10.3791/55143.

Abstract

Detection of genomic changes at single cell resolution is important for characterizing genetic heterogeneity and evolution in normal tissues, cancers, and microbial populations. Traditional methods for assessing genetic heterogeneity have been limited by low resolution, low sensitivity, and/or low specificity. Single cell sequencing has emerged as a powerful tool for detecting genetic heterogeneity with high resolution, high sensitivity and, when appropriately analyzed, high specificity. Here we provide a protocol for the isolation, whole genome amplification, sequencing, and analysis of single cells. Our approach allows for the reliable identification of megabase-scale copy number variants in single cells. However, aspects of this protocol can also be applied to investigate other types of genetic alterations in single cells.

Publication types

  • Video-Audio Media

MeSH terms

  • DNA Copy Number Variations*
  • Genome
  • Genomics / methods*
  • High-Throughput Nucleotide Sequencing / methods*
  • Humans
  • Markov Chains
  • Neoplasms / genetics
  • Sensitivity and Specificity
  • Single-Cell Analysis / methods*