High-density speckle contrast optical tomography (SCOT) for three dimensional tomographic imaging of the small animal brain

Tanja Dragojević; Hari M Varma; Joseph L Hollmann; Claudia P Valdes; Joseph P Culver; Carles Justicia; Turgut Durduran

doi:10.1016/j.neuroimage.2017.04.003

High-density speckle contrast optical tomography (SCOT) for three dimensional tomographic imaging of the small animal brain

Neuroimage. 2017 Jun:153:283-292. doi: 10.1016/j.neuroimage.2017.04.003. Epub 2017 Apr 5.

Authors

Tanja Dragojević¹, Hari M Varma², Joseph L Hollmann², Claudia P Valdes², Joseph P Culver³, Carles Justicia⁴, Turgut Durduran⁵

Affiliations

¹ ICFO-Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, 08860 Castelldefels, Barcelona, Spain. Electronic address: [email protected].
² ICFO-Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, 08860 Castelldefels, Barcelona, Spain.
³ Department of Radiology, Washington University School of Medicine,St. Louis, MO 63110, USA; Department of Physics, Washington University, St. Louis, MO 63130, USA.
⁴ Department of Brain Ischemia and Neurodegeneration, Insitut d'Investigacions Biomèdiques de Barcelona (IIBB), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain; Àrea de Neurociències, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
⁵ ICFO-Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, 08860 Castelldefels, Barcelona, Spain; Institució Catalana de Recerca i Estudis Avançats (ICREA), 08015 Barcelona, Spain.

PMID: 28389382
DOI: 10.1016/j.neuroimage.2017.04.003

Abstract

High-density speckle contrast optical tomography (SCOT) utilizing tens of thousands of source-detector pairs, was developed for in vivo imaging of blood flow in small animals. The reduction in cerebral blood flow (CBF) due to local ischemic stroke in a mouse brain was transcanially imaged and reconstructed in three dimensions. The reconstructed volume was then compared with corresponding magnetic resonance images demonstrating that the volume of reduced CBF agrees with the infarct zone at twenty-four hours.

Keywords: (110.3010) Image reconstruction techniques; (110.6150) Speckle imaging; (170.3880) Medical and biological imaging; OCIS codes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Algorithms
Animals
Brain Ischemia / diagnostic imaging*
Brain Ischemia / physiopathology
Cerebral Cortex / blood supply*
Cerebral Cortex / physiopathology
Image Processing, Computer-Assisted
Magnetic Resonance Imaging
Male
Mice, Inbred C57BL
Signal Processing, Computer-Assisted
Stroke / diagnostic imaging*
Stroke / physiopathology
Tomography, Optical / methods*