The protective effect of platelet released growth factors and bone augmentation (Bio-Oss®) on ethanol impaired osteoblasts

Tolga Taha Sönmez; Andreas Bayer; Tillman Cremer; Jennifer Vanessa Phi Hock; Bernd Lethaus; Nisreen Kweider; Christoph Jan Wruck; Wolf Drescher; Holger Jahr; Sebastian Lippross; Thomas Pufe; Mersedeh Tohidnezhad

doi:10.1016/j.aanat.2017.07.002

The protective effect of platelet released growth factors and bone augmentation (Bio-Oss^®) on ethanol impaired osteoblasts

Ann Anat. 2017 Nov:214:36-42. doi: 10.1016/j.aanat.2017.07.002. Epub 2017 Jul 31.

Authors

Affiliations

¹ Department of Oral and Maxillofacial Surgery, Medical Faculty, RWTH Aachen University, Pauwelsstr. 30, 52074 Aachen, Germany; Institute of Anatomy and Cell Biology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany. Electronic address: [email protected].
² Department of Heart and Vascular Surgery, University Hospital of Schleswig-Holstein, Kiel, Germany. Electronic address: [email protected].
³ Institute of Anatomy and Cell Biology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany. Electronic address: [email protected].
⁴ Institute of Anatomy and Cell Biology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany. Electronic address: [email protected].
⁵ Department of Oral and Maxillofacial Surgery, Medical Faculty, RWTH Aachen University, Pauwelsstr. 30, 52074 Aachen, Germany. Electronic address: [email protected].
⁶ Institute of Anatomy and Cell Biology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany. Electronic address: [email protected].
⁷ Institute of Anatomy and Cell Biology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany.
⁸ Department of Orthopedic Surgery of the Lower Limb and Arthroplasty, Rummelsberg Hospital, Schwarzenbruck, Germany. Electronic address: [email protected].
⁹ Department of Orthopedic Surgery, RWTH Aachen University, Pauwelsstr. 30, D-52074 Aachen, Germany. Electronic address: [email protected].
¹⁰ Department of Traumatology, University Hospital of Schleswig-Holstein, Kiel, Germany. Electronic address: [email protected].
¹¹ Institute of Anatomy and Cell Biology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany. Electronic address: [email protected].
¹² Institute of Anatomy and Cell Biology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany. Electronic address: [email protected].

PMID: 28774818
DOI: 10.1016/j.aanat.2017.07.002

Abstract

Background: Chronic alcohol consumption is a known limiting factor for bone healing. One promising strategy to improve bone augmentation techniques with Bio-Oss^® in oral and maxillofacial surgery might be the supportive application of platelet-concentrated biomaterials as platelet-released growth factor (PRGF). To address this matter, we performed an in vitro study investigating the protective effects of PRGF and Bio-Oss^® in ethanol (EtOH) treated osteoblasts.

Methods: The SAOS-2 osteosarcoma cell line, with and without EtOH pretreatment was used. The cell viability, proliferation and alkali phosphatase activity (ALP) after application of 0%, 5% and 10% PRGF and Bio-Oss^® were assessed.

Results: The application of PRGF and Bio-Oss^® in EtOH impaired osteoblasts showed a significant beneficial influence increasing the viability of the osteoblasts in cell culture. The synergistic effect of Bio-Oss^® and 5% PRGF on the proliferation of osteoblasts was also demonstrated. Bio-Oss^® only in combination with PRGF increases the alkaline phosphatase (ALP) activity in EtOH pretreated cells.

Conclusions: These results indicate that the simultaneous application of PRGF and Bio-Oss^® inhibits EtOH induced bone healing impairment. Furthermore, in the cells, PRGF induced a protective mechanism which might promote bone regeneration.

Keywords: Bio-Oss(®); Ethanol; Osteoblasts; Platelet released growth factor.

MeSH terms

Blood Platelets / metabolism*
Bone Substitutes / administration & dosage
Cell Line
Cell Proliferation / drug effects*
Cell Proliferation / physiology
Cell Survival / drug effects*
Cell Survival / physiology
Cytoprotection / drug effects
Dose-Response Relationship, Drug
Drug Combinations
Ethanol / toxicity*
Humans
Intercellular Signaling Peptides and Proteins / administration & dosage*
Minerals / administration & dosage*
Osteoblasts / cytology
Osteoblasts / drug effects*
Osteoblasts / physiology
Treatment Outcome

Substances

Bio-Oss
Bone Substitutes
Drug Combinations
Intercellular Signaling Peptides and Proteins
Minerals
Ethanol