Optimizing Blood Collection and Processing for Quantiferon-TB Gold in-Tube Testing Gives Low Rates of Indeterminate Results: Clinical Implications

Faisal Kordy; Valerie Waters; Cathy Den-Hollander; Stanley Read; Ray Lam; Ian Kitai

doi:10.1097/INF.0000000000001732

Optimizing Blood Collection and Processing for Quantiferon-TB Gold in-Tube Testing Gives Low Rates of Indeterminate Results: Clinical Implications

Pediatr Infect Dis J. 2018 Jan;37(1):e22-e24. doi: 10.1097/INF.0000000000001732.

Authors

Faisal Kordy, Valerie Waters, Cathy Den-Hollander¹, Stanley Read, Ray Lam, Ian Kitai

Affiliation

¹ Department of Paediatrics, Division of Infectious Diseases, The Hospital for Sick Children, Department of Paediatrics, and Department of Paediatric Laboratory Medicine, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada.

PMID: 28787385
DOI: 10.1097/INF.0000000000001732

Abstract

The rate of low-mitogen indeterminate interferon-gamma release assay results at a hospital with expert pediatric phlebotomy and rapid incubation of specimens was 0.96%. All low-mitogen indeterminate results were found to be associated with an immunocompromised or anergic state. We describe a child where an unexpected indeterminate interferon-gamma release assay test pointed to an underlying anergic condition and was of diagnostic significance.

Publication types

Case Reports

MeSH terms

Adolescent
Anti-Inflammatory Agents / therapeutic use
Antitubercular Agents / therapeutic use
Blood Specimen Collection* / methods
Blood Specimen Collection* / standards
Case-Control Studies
Child
Child, Preschool
Female
Humans
Infliximab / therapeutic use
Interferon-gamma Release Tests* / methods
Interferon-gamma Release Tests* / standards
Male
Tuberculosis / diagnosis*
Tuberculosis / drug therapy
Tuberculosis / immunology

Substances

Anti-Inflammatory Agents
Antitubercular Agents
Infliximab