Cytoplasmic Lipid Accumulation Characteristic of the Cribriform Variant of Papillary Thyroid Carcinoma

Pathobiology. 2017;84(5):251-257. doi: 10.1159/000477910. Epub 2017 Aug 16.

Abstract

Objective: The purpose of this study was to clarify the diagnostic significance of cytoplasmic lipid accumulation (CLIA) in the cribriform variant of papillary thyroid carcinoma (CV-PTC).

Methods: We performed a histological, immunohistochemical, and cytological examination of 35 CV-PTC cases at the Kuma Hospital. CLIA was defined as bubble-like multivacuolation in cytoplasm with distinct cell border. We also examined 100 conventional PTC (con-PTC) cases as controls.

Results: Histological analysis showed the presence of carcinoma cells with CLIA in 60.0% of CV-PTC and 5.0% of con-PTC cases. The vacuoles tended to distribute in the subnuclear portion of carcinoma cells showing papillary growth. They were positive for oil red O staining and adipophilin. The carcinoma cells without the vacuoles showed a subnuclear dot-like expression for adipophilin in CV-PTC cases, but not in the con-PTC cases. Cytological analysis showed CLIA in 17 (54.8%) of the 31 CV-PTC cases, but not in the con-PTC cases.

Conclusion: This is the first study to report the presence of carcinoma cells with CLIA in CV-PTC. The subnuclear dot-like expression of adipophilin may be characteristic of CV-PTC. These findings might be related to degenerative changes occurring in CV-PTC.

Keywords: Adipophilin; Cribriform variant; Foamy cytoplasm; Papillary thyroid carcinoma; Subnuclear lipid accumulation.

MeSH terms

  • Adenocarcinoma / diagnosis
  • Adenocarcinoma / pathology*
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Azo Compounds / chemistry
  • Carcinoma, Papillary / classification
  • Carcinoma, Papillary / diagnosis
  • Carcinoma, Papillary / pathology*
  • Cytoplasm / chemistry*
  • Cytoplasm / ultrastructure
  • Female
  • Histological Techniques
  • Humans
  • Immunohistochemistry
  • Lipids / physiology*
  • Male
  • Middle Aged
  • Perilipin-2 / chemistry
  • Retrospective Studies
  • Thyroid Cancer, Papillary
  • Thyroid Neoplasms / classification
  • Thyroid Neoplasms / diagnosis
  • Thyroid Neoplasms / pathology*
  • Young Adult

Substances

  • Azo Compounds
  • Lipids
  • Perilipin-2
  • oil red O