Evaluation of inositol phosphates in urine after topical administration of myo-inositol hexaphosphate to female Wistar rats

Aims: Previous studies demonstrated a remarkable increase of urinary InsP₆ by topical administration. However, the methodology used for InsP₆ analysis was not specific. The aim of this paper is to measure urinary inositol phosphates InsPs using more advanced methodologies and to compare the results with those obtained by the non-specific method.

Materials and methods: We fed 12 female rats with a diet without InsP₆ for 16days. Then, we administered a topical InsP₆ gel at high doses for 7days (50mgInsP₆/day) or at low doses for 28days (20mgInsP₆/day). We measured urine levels InsPs using a nonspecific method (based on the ability of InsPs to complex Al³⁺) and levels of InsP₆ by a specific method (using polyacrylamide gel electrophoresis). Identification of different InsPs was performed by MS.

Key findings: At baseline, after dietary deprivation of InsP₆, rats only excreted InsP₂ in their urine, and there was no detectable InsP₆ or other InsPs. Rats given the high dose treatment for 7days had abundant urinary InsP₆, but also had other InsPs in their urine; cessation of InsP₆ administration led to decreased levels of urinary InsPs. Rats given the low dose treatment for 28days had increasing levels of urinary InsPs over time. The maximum urinary InsP₆ was at 21days, after which InsPs excretion decreased.

Significance: We conclude that the skin can absorb InsP₆ from a topical gel, and that InsP₆ is excreted in the urine, along with other InsPs (InsP₅, InsP₄, InsP₃, and InsP₂).